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KMID : 1007520130220020593
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Food Science and Biotechnology
2013 Volume.22 No. 2 p.593 ~ p.598
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Antiviral Activity of Green Tea Catechins against Feline Calicivirus as a Surrogate for Norovirus
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Oh Eun-Gyoung
Kim Kyoung-Lan
Shin Soon-Bum
Son Kwang-Tae
Lee Hee-Jung
Kim Tae-Hoon
Kim Young-Mog
Cho Eun-Ji
Kim Do-Kyun
Lee Eun-Woo
Lee Myung-Suk
Shin Il-Shik
Kim Ji-Hoe
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Abstract
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In this study, several medicinal plant extracts including spices, herb teas, and medical herbs were screened for antiviral activity against feline calicivirus (FCV), a surrogate of norovirus. Among them, a methanolic extract of green tea, Camellia sinensis, only exhibited a significant antiviral activity against FCV. The methanolic extract was further fractionated with several solvents such as methanol (MeOH), n-hexane, chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (n-BuOH), and water. EtOAc-soluble fraction exhibited the highest antiviral activity against FCV. Moreover, the analysis of the most active fraction using a HPLC led to the identification of 4 known catechins: (-)-epigallocatechin (EGC), (-)-epicatechin (EC), (-)-epigallocatechin gallate (EGCG), and (-)-epicatechin gallate (ECG). Among the catechins tested in this study, EGCG exhibited the most effective antiviral activity (EC50, 12 mg/mL) with relatively low cytotoxicity (CC50, 320 mg/ mL), resulting relatively high selectivity index value 26.67. To our knowledge, this is the first experimental verification showing antiviral activity of catechins from green tea against FCV.
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KEYWORD
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antiviral activity, Camellia sinensis, catechin, feline calicivirus, norovirus
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